TUBB3 Antikörper (Tubulin, Beta, 3) (AA 443-450)

Details for Product anti-TUBB3 Antibody No. ABIN1742553, Anbieter: Anmelden zum Anzeigen
Antigen
  • tubb3
  • TUBB3
  • TUBB4
  • CDCBM
  • CFEOM3A
  • beta-4
  • 3200002H15Rik
  • M(beta)3
  • M(beta)6
  • AI325297
  • M(beta)4
  • Tubb
  • Tubb4
  • tubulin beta 3 class III
  • tubulin beta 3 class III L homeolog
  • beta-tubulin 3
  • tubulin beta-4A chain-like
  • tubulin beta-3 chain pseudogene
  • tubulin beta-3 chain
  • beta tubulin 3
  • tubulin beta 4B class IVb
  • tubulin, beta 3 class III
  • tubulin, beta 4A class IVA
  • tubb3
  • tubb3.L
  • LOC698646
  • TUBB3
  • LOC700985
  • LOC100581245
  • tub3
  • TUBB4B
  • Tubb3
  • Tubb4a
Epitop
AA 443-450
47
47
20
15
13
6
5
4
4
4
3
3
3
3
2
2
1
1
1
1
Reaktivität
Human, Ratte (Rattus), Maus
249
120
108
23
20
15
15
8
8
6
6
5
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
Wirt
Kaninchen
135
125
20
15
1
Klonalität
Polyklonal
Konjugat
Dieser TUBB3 Antikörper ist unkonjugiert
24
13
12
5
5
5
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
250
109
80
68
67
58
54
18
13
13
12
8
8
6
2
2
2
Optionen
Hersteller
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Hersteller Produkt- Nr.
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'Independent Validation' Siegel
Chargennummer 302302/3
Validierte Anwendung Western Blotting
Positivkontrolle Mouse brain
Negativkontrolle Mouse heart, HeLa cells, A431 cells
Bewertung A band was observed in the positive controls at the expected size (~50 kDa), which is lower in the negative controls.
Primärantikörper
Sekundärantikörper
Kontrollen - Lysates were prepared by Kinexus Bioinformatics Corporation following standard protocols and quality controlled for protein integrity on a regular basis.
Protokoll
  • 1. Cell/tissue total protein lysates were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 minutes prior to loading.
  • 2. 15 μg of boiled lysate were loaded and resolved on a 12% SDS-polyacrylamide gel.
  • 3. The Precision Plus Protein™ All Blue Prestained Standards from BioRad (161-0373) were used as molecular mass markers.
  • 4. Proteins were transferred onto nitrocellulose membrane by tank transfer and protein transfer was confirmed with Ponceau S staining.
  • 5. The immunoblot membrane was blocked in 2.5% skim milk and 1.5% BSA solution in TTBS at room temperature for 60 minutes.
  • 6. The membrane was washed in TTBS twice for 5 minutes each.
  • 7. The membrane was immersed with the protein side up in the antibody solution in TBS and incubated overnight at 4°C with gentle agitation.
  • 8. The membrane was rinsed twice with TTBS.
  • 9. The membrane was washed in TTBS twice for 5 minutes each.
  • 10. The membrane was washed in TTBS once for 15 minutes.
  • 11. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBS for 60 minutes at room temperature with gentle agitation.
  • 12. The membrane was rinsed twice with TTBS.
  • 13. The membrane was washed in TTBS twice for 5 minutes each.
  • 14. The membrane was washed in TTBS once for 15 minutes.
  • 15. Signals were detected by chemiluminescence (ECL). The blot was scanned for 320 seconds.
  • 16. The membrane was rinsed three times with TTBS.
  • 17. Repeated Steps 4-15 with the loading control antibody and its matching secondary antibody. The blot was scanned for 160 seconds.
Anmerkungen - No experimental challenges noted.
Validierungsbilder
Western Blotting validation image for anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody (ABIN1742553) Figure 1: Western Blot for Tubulin, Beta, 3 (TUBB3). Grey arrowhead indicates the exp...
Immunogen Synthetic peptide (aa 443-450 of mouse beta3-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.
Spezifität Specific for beta3-tubulin.
Reinigung antiserum
Andere Bezeichnung beta3-Tubulin (TUBB3) (TUBB3 Antibody Abstract)
Hintergrund Synonyms: TuJ1
Pathways Microtubule Dynamics, M Phase
Applikationshinweise WB: 1 : 1000 up to 1 : 10000 (AP staining)
ICC: 1 : 1000 up to 1 : 5000
IHC: 1 : 200 up to 1 : 500
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer PBS
Handhabung Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Bilder des Herstellers
Western Blotting (WB) image for anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody (ABIN1742553) anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody
Immunocytochemistry (ICC) image for anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody (ABIN1742553) anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody (ABIN1742553) anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody (Image 3)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tubulin, Beta, 3 (TUBB3) (AA 443-450) antibody (ABIN1742553) Indirect immunofluorescence labeling of PFA fixed, paraffin embedded mouse brain sect...
Produkt verwendet in: Kaczor, Rakus, Mozrzymas: "Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes." in: Frontiers in cellular neuroscience, Vol. 9, pp. 120, 2015 (PubMed).

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