His Tag Antikörper (HRP)

Details zu Produkt Nr. ABIN119352, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
612
26
15
3
3
Wirt
Maus
410
185
27
23
4
4
3
1
Klonalität (Klon)
Monoklonal ()
Konjugat
HRP
57
45
36
14
13
11
10
10
9
9
9
9
8
8
8
7
7
7
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Western Blotting (WB)
549
389
164
109
78
78
74
26
13
10
9
7
6
6
6
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen PAX6 transcription factor linked to histidine tag. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Sequenz HHHHHH
Klon AD1-1-10
Isotyp IgG1
Reinigung Affinity Chromatography on Protein G
Andere Bezeichnung 6xHistidine Epitope Tag
Substanzklasse Tag
Hintergrund 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.
Applikationshinweise ELISA: 1/20 - 1/1000. Western Blot: 1/1000 - 1/5000, In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.01 % Thiomersal as preservative and a HRP stabilizer
Lagerung 4 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Bilder des Herstellers
Western Blotting (WB) image for anti-His Tag antibody (HRP) (ABIN119352) Western blot analysis of His-Ubiquitin recombinant protein probed with MOUSE ANTI HIS...
Allgemeine Veröffentlichungen Diefenbacher, Thorsteinsdottir, Spang: "The Dsl1 tethering complex actively participates in soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor (SNARE) complex assembly at the endoplasmic reticulum in Saccharomyces cerevisiae." in: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 25027-38, 2011 (PubMed).

Alvarez, López-Pacheco, Aguilar-Yañez, Portillo-Lara, Mendoza-Ochoa, García-Echauri, Freiden, Schultz-Cherry, Zertuche-Guerra, Bulnes-Abundis, Salgado-Gallegos, Elizondo-Montemayor, Hernández-Torre: "Specific recognition of influenza A/H1N1/2009 antibodies in human serum: a simple virus-free ELISA method." in: PLoS ONE, Vol. 5, Issue 4, pp. e10176, 2010 (PubMed).

Gunnarsen, Lunde, Kristiansen, Bogen, Sandlie, Løset: "Periplasmic expression of soluble single chain T cell receptors is rescued by the chaperone FkpA." in: BMC biotechnology, Vol. 10, pp. 8, 2010 (PubMed).

Wrighton, Lin, Yu, Feng: "Transforming Growth Factor {beta} Can Stimulate Smad1 Phosphorylation Independently of Bone Morphogenic Protein Receptors." in: The Journal of biological chemistry, Vol. 284, Issue 15, pp. 9755-63, 2009 (PubMed).

Bahi, Dreyer: "Overexpression of plasminogen activators in the nucleus accumbens enhances cocaine-, amphetamine- and morphine-induced reward and behavioral sensitization." in: Genes, brain, and behavior, Vol. 7, Issue 2, pp. 244-56, 2008 (PubMed).

Hwang, Duvall, Tomlinson, Boackle et al.: "Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable ..." in: Molecular immunology, Vol. 45, Issue 9, pp. 2570-80, 2008 (PubMed).

Manno: "Measurement of the digit lengths and the anogenital distance in mice." in: Physiology & behavior, Vol. 93, Issue 1-2, pp. 364-8, 2008 (PubMed).

Hoffmann, Schellack, Textor, Konold, Schmitz, Cerwenka, Pflanz, Watzl: "Identification of CLEC12B, an inhibitory receptor on myeloid cells." in: The Journal of biological chemistry, Vol. 282, Issue 31, pp. 22370-5, 2007 (PubMed).

Zheng, Kohler, Chen, Weber, Khan, Johnson, Orentas: "Serum from mice immunized in the context of Treg inhibition identifies DEK as a neuroblastoma tumor antigen." in: BMC immunology, Vol. 8, pp. 4, 2007 (PubMed).

Els Conrath, Lauwereys, Wyns, Muyldermans: "Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7346-50, 2001 (PubMed).

Suen, Wu, Chen, Wu, Chiang: "Characterization of self-T-cell response and antigenic determinants of U1A protein with bone marrow-derived dendritic cells in NZB x NZW F1 mice." in: Immunology, Vol. 103, Issue 3, pp. 301-9, 2001 (PubMed).

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