Myeloperoxidase Antikörper

Produktnummer ABIN119048

Produktdetails anti-Myeloperoxidase Antikörper

Target: Myeloperoxidase (MPO)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Myeloperoxidase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Human Myeloperoxidase. Spleen cells from immunised mice were fused with cells of the mouse X63 AG8653 myeloma cell line.

Klon: 2C7

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry (1/50-1/100): Membrane permeabilisation is required. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry. Immunhistology on Frozen Sections (1/1000-1/5000). Immunhistology on Paraffin Sections (1/500-1/1000): use bone marrow as positive control. ELISA. Western blotting (Non reducing conditions): ABIN119048 recognises native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognises recombinant MPO in Western blots and weakly in ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-Myeloperoxidase Antikörper (ABIN119048)

OBrien, Abdulahad, Rutgers, Huitema, OReilly, Coughlan, Harrington, Heeringa, Little, Hickey: "Intermediate monocytes in ANCA vasculitis: increased surface expression of ANCA autoantigens and IL-1β secretion in response to anti-MPO antibodies." in: Scientific reports, Vol. 5, pp. 11888, (2015) (PubMed).

Schreiber, Xiao, Jennette, Schneider, Luft, Kettritz: "C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis." in: Journal of the American Society of Nephrology : JASN, Vol. 20, Issue 2, pp. 289-98, (2009) (PubMed).

Details zu Myeloperoxidase

Target: Myeloperoxidase (MPO)

Andere Bezeichnung: Myeloperoxidase (MPO Produkte)

Synonyme: mKIAA4033 antikoerper, MPO antikoerper, LOC100335032 antikoerper, POX2' antikoerper, XPOX2' antikoerper, mpo antikoerper, mpo-A antikoerper, pmr-1 antikoerper, pmr1 antikoerper, pox2 antikoerper, xpox2 antikoerper, myeloperoxidase antikoerper, myeloperoxidase L homeolog antikoerper, MPO antikoerper, Mpo antikoerper, LOC100335032 antikoerper, mpo.L antikoerper

Hintergrund: Myeloperoxidase/MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55 kD) and two light chains (15 kD), the heavy chains being linked by a disulphide bond. Myeloperoxidase is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59-64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2. Traditionally Myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-Myeloperoxidase auto-antibody levels are also reported in rheumatoid arthritis. Recently it was shown that Myeloperoxidase participates in the initiation and progression of cardiovascular disease. It possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers.Synonyms: MPO

Gen-ID: 9606

UniProt: P05164

Pathways: Chromatin Binding