PTK2B Antikörper (PTK2B Protein tyrosine Kinase 2 beta) (pTyr402) (Alexa Fluor 647)

Details for Product anti-PTK2B Antibody No. ABIN1177155, Anbieter: Anmelden zum Anzeigen
Antigen
  • Akt
  • PKB
  • Rac
  • PKB/Akt
  • PKBalpha
  • CADTK
  • CAKB
  • FADK2
  • FAK2
  • PTK
  • PYK2
  • RAFTK
  • CAKbeta
  • Pyk2
  • E430023O05Rik
  • Raftk
  • v-akt murine thymoma viral oncogene homolog 1
  • thymoma viral proto-oncogene 1
  • protein tyrosine kinase 2 beta
  • PTK2 protein tyrosine kinase 2 beta
  • PTK2B protein tyrosine kinase 2 beta
  • Akt1
  • PTK2B
  • Ptk2b
Epitop
pTyr402
53
20
15
15
14
13
13
10
8
8
7
7
5
5
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
254
166
146
12
11
9
7
7
6
5
2
1
1
1
Wirt
Maus
214
41
3
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser PTK2B Antikörper ist konjugiert mit Alexa Fluor 647
6
6
5
3
3
3
3
3
3
3
3
3
3
3
3
2
2
Applikation
Intracellular Staining (ICS)
202
105
95
57
39
25
21
6
3
2
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Marke BD Phosflow™
Immunogen Phosphorylated Human Pyk2 Peptide
Klon L68-1256-272
Isotyp IgG2b, kappa
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Andere Bezeichnung Pyk2 (PTK2B Antibody Abstract)
Hintergrund Proline-rich tyrosine kinase 2 (Pyk2) is a member of the FAK subfamily of tyrosine protein kinases. Pyk2 responds to a wide variety of inflammatory and stress stimuli, such as cytokines, ligation of integrins or antigen receptors, mechanical strain, and cellular depolarization. A common characteristic of most Pyk2 stimuli is an increase in cytosolic free calcium. Pyk2 is involved in signaling pathways that regulate vital cellular functions in hematopoietic, epithelial, neuronal, endothelial, and other cell types. Activation of Pyk2 initiates its autophosphorylation at tyrosine 402 (Y402), followed by Src-mediated phosphorylation of other Pyk2 tyrosine sites, which enhances its activity and leads to downstream signal transduction through the MAPK and JNK pathways. The L68-1256.272 monoclonal antibody recognizes the phosphorylated Y402 of activated Pyk2.
Synonyms: Protein tyrosine kinase 2beta, FAK2, FADK 2, CAKbeta, CADTK, RAFTK
Pathways EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation
Applikationshinweise Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.
Probenmenge 20 μL
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Produkt verwendet in: Anand, Cucchiarini, Terwilliger, Ganju: "The tyrosine kinase Pyk2 mediates lipopolysaccharide-induced IL-8 expression in human endothelial cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 8, pp. 5636-44, 2008 (PubMed).

Faure, Corvol, Toutant, Valjent, Hvalby, Jensen, El Messari, Corsi, Kadaré, Girault: "Calcineurin is essential for depolarization-induced nuclear translocation and tyrosine phosphorylation of PYK2 in neurons." in: Journal of cell science, Vol. 120, Issue Pt 17, pp. 3034-44, 2007 (PubMed).

Gluck: "Acid sensing in renal epithelial cells." in: The Journal of clinical investigation, Vol. 114, Issue 12, pp. 1696-9, 2004 (PubMed).

Orr, Murphy-Ullrich: "Regulation of endothelial cell function BY FAK and PYK2." in: Frontiers in bioscience : a journal and virtual library, Vol. 9, pp. 1254-66, 2004 (PubMed).

Park, Avraham, Avraham: "RAFTK/Pyk2 activation is mediated by trans-acting autophosphorylation in a Src-independent manner." in: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33315-22, 2004 (PubMed).

Avraham, Park, Schinkmann, Avraham: "RAFTK/Pyk2-mediated cellular signalling." in: Cellular signalling, Vol. 12, Issue 3, pp. 123-33, 2000 (PubMed).

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