IFNB1 Antikörper

Produktnummer ABIN116507

Der Kaninchen Polyklonal anti-IFNB1 Antikörper wird verwendet zum Nachweis von IFNB1 in Proben von Human. Er wurde validiert für WB, IHC (p), EIA und Func.

Kurzübersicht für IFNB1 Antikörper (ABIN116507)

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IFNB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-IFNB1 Antikörper

Spezifität: This antibody detects Interferon-beta (IFN-beta).

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Antigen Affinity Chromatography using immobilized IFN-beta matrix

Immunogen: Highly purified (>98%) E.coli derived recombinant Human Inferferon beta

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman IFN-beta (1.0 ng/mL), a concentration of 0.015-0.025 μg/mL of this antibody isrequired. Indirect ELISA: To detect Human IFN-beta by Indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial before opening.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu IFNB1

Target: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Andere Bezeichnung: IFNB / Interferon beta

Hintergrund: The mammalian type I Inteferons (IFN1) are produced in response to viral infection and other inducers. They are divided into alpha and beta subtypes leukocytes and fibroblasts reactivity. The human IFN alphas are encoded by a family of at least 15 different genes, while IFN beta is the unique member of its subtype. There is approximately 50 % amino acid homology between the alpha and beta subtypes. Both IFN subtypes are pleiotropic cytokines and have a similar range of biological activities. Differences between alpha subtypes, and between IFN alpha and betas, are in potency and cell type specific activities. In particular, IFN beta elicits a markedly higher antiproliferation response in some cell types such as, embryonal carcinoma, melanoma and melanocytes than do IFN alphas. Higher potency of IFN beta in treatment of multiple sclerosis and certain cancers has been observed. Type I IFNs signal through binding to a common cell surface receptor. Two chains of the receptor, IFNAR1 and IFNAR2, have been identified. Both chains are necessary for function and in the absence of either there is neither high affinity binding nor biological activity. The intracellular portions of the receptor subunits are bound by tyrosine kinases, Jak1 and Tyk2, members of the Janus kinase family. Upon ligand binding these kinases are activated and phosphorylate members of the STAT family of transcription factors, as well as IFNAR1 and 2.Synonyms: Fibroblast interferon, IFB, IFN-beta, IFNB1

Gen-ID: 3456

NCBI Accession: NP_002167

UniProt: P01574

Pathways: JAK-STAT Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagie, Inflammasome