Thrombopoietin Antikörper

Produktnummer ABIN116255

Produktdetails anti-Thrombopoietin Antikörper

Target: Thrombopoietin (THPO)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Thrombopoietin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Spezifität: This antibody reacts with Thrombopoietin.

Aufreinigung: Affinity chromatography.

Immunogen: Highly purified recombinant human TPO.

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTPO (5.00 ng/mL), a concentration of 4.2-6.3 μg/mL of this antibody is required. ELISA: (Indirect): To detect hTPO by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTPO. (Sandwich): To detect hTPO by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TPO (PP1074B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTPO is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu Thrombopoietin

Target: Thrombopoietin (THPO)

Andere Bezeichnung: Thrombopoietin (THPO Produkte)

Synonyme: MGDF antikoerper, MKCSF antikoerper, ML antikoerper, MPLLG antikoerper, THCYT1 antikoerper, TPO antikoerper, Mgdf antikoerper, Ml antikoerper, Mpllg antikoerper, Tpo antikoerper, Tpo1 antikoerper, Tpo2 antikoerper, Tpo3 antikoerper, Tpo4 antikoerper, tpo antikoerper, LOC100231762 antikoerper, thrombopoietin antikoerper, THPO antikoerper, Thpo antikoerper, thpo antikoerper

Hintergrund: Synonyms: C-mpl ligand, MGDF, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, THPO

Gen-ID: 7066

NCBI Accession: NP_000451

UniProt: P40225

Pathways: JAK-STAT Signalweg, Hormone Activity