KI-67 Antikörper (Antigen Identified By Monoclonal Antikörper Ki-67)

Details for Product anti-MKI67 Antibody No. ABIN115329, Anbieter: Anmelden zum Anzeigen
Antigen
  • MKI67
  • kia
  • mki67
  • MGC132156
  • D630048A14Rik
  • Ki-67
  • Ki67
  • KIA
  • MIB-1
  • antigen identified by monoclonal antibody Ki-67
  • antigen identified by monoclonal antibody Ki 67
  • MKI67
  • mki67
  • Mki67
Reaktivität
Human
457
113
72
17
16
9
4
2
1
Wirt
Kaninchen
260
158
32
9
5
3
Klonalität
Polyklonal
Konjugat
Dieser KI-67 Antikörper ist unkonjugiert
17
13
11
10
10
8
7
6
5
4
4
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
187
184
158
120
119
105
85
72
16
13
13
11
6
3
3
3
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Synthetic peptide from 62 base pair region of the human Ki-67 antigen.
Spezifität This antibody reacts with a nuclear antigen present in proliferating human cells. It can be used to evaluate the ki-67 labeling index in various tumors.
Cellular Localization: Nuclear.
Reinigung Purified
Andere Bezeichnung Ki-67 (MKI67 Antibody Abstract)
Hintergrund Ki-67 antigen is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki-67 gene contains 15 exons. The Ki-67 repeat region, within which there is a 22-amino acid Ki-67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis re-vealed multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quies-cent cells. Immunoblot analysis showed expression of 320- and 359-kD proteins. Sequence analy-sis predicted that the short-lived 2,896- and 3,256-amino acid protein isoforms contain potential nuclear targeting signals, over 200 potential phosphorylation sites, 19 N-myristoylation sites, 3 amidation sites, and numerous PEST sites. Antisense oligonucleotides inhibited cellular prolifera-tion in a dose-dependent manner, suggesting that Ki-67 protein expression may be an absolute requirement for cell proliferation.Synonyms: Ki67 antigen, MKI67, Proliferation marker
Gen-ID 4288
NCBI Accession NP_001139438
UniProt P46013
Forschungsgebiet Proliferation Markers, Cell Cycle
Pathways Glycosaminoglycan Metabolic Process
Applikationshinweise Immunohistochemsistry on Frozen and Formalin-Fixed Paraffin Embedded Sections: Use adilution of 1/25-1/50 in an ABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) antibody (ABIN115329) Formalin fixed paraffin embedded human tonsil stained with Ki-67 antibody. ABIN115328
Allgemeine Veröffentlichungen Wintzer, Zipfel, Schulte-Moenting, Hellerich, von Kleist: "Ki-67 immunostaining in human breast tumors and its relationship to prognosis." in: Cancer, Vol. 67, Issue 2, pp. 421-8, 1991 (PubMed).

Brown, Gatter: "Monoclonal antibody Ki-67: its use in histopathology." in: Histopathology, Vol. 17, Issue 6, pp. 489-503, 1991 (PubMed).

Gerdes, Li, Schlueter, Duchrow, Wohlenberg, Gerlach, Stahmer, Kloth, Brandt, Flad: "Immunobiochemical and molecular biologic characterization of the cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki-67." in: The American journal of pathology, Vol. 138, Issue 4, pp. 867-73, 1991 (PubMed).

Gerdes: "Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies." in: Seminars in cancer biology, Vol. 1, Issue 3, pp. 199-206, 1991 (PubMed).

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