TJP1 Antikörper (Tight Junction Protein 1 (Zona Occludens 1)) (AA 886-995)

Details for Product anti-TJP1 Antibody No. ABIN1109613, Anbieter: Anmelden zum Anzeigen
Antigen
  • ZO-1
  • ZO1
  • zo1
  • tight junction protein 1
  • tight junction protein 1 (zona occludens 1)
  • zonula occludens 1
  • TJP1
  • Tjp1
  • ZO1
Alternativen
anti-Ratte (Rattus) TJP1 Antikörper für ELISA
Epitop
AA 886-995
16
10
5
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reaktivität
Hund, Human, Maus, Ratte (Rattus)
90
32
32
20
10
5
4
4
3
2
1
1
1
1
Wirt
Kaninchen
72
16
3
3
2
Klonalität
Polyklonal
Konjugat
Dieser TJP1 Antikörper ist unkonjugiert
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
72
24
23
23
23
14
13
10
10
8
7
3
2
1
1
Optionen
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Immunogen Amino acids 886-995 of human ZO-1
Isotyp Ig
Spezifität This antibody recognizes specifically ZO-1 alpha-plus.
Kreuzreaktivität (Details) Species reactivity (tested):Human, Mouse, Rat, Dog.
Reinigung Protein A Chromatography
Andere Bezeichnung ZO-1 / Tight Junction Protein 1 (TJP1 Antibody Abstract)
Hintergrund Zona occludens 1 (ZO-1) is an ~220 kDa tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.Synonyms: Tjp1, Zo1, Zona occludens protein 1, Zonula occludens protein 1
Gen-ID 7082
NCBI Accession NP_003248
UniProt Q07157
Pathways Carbohydrate Homeostasis, Cell-Cell Junction Organization
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.1 mg/mL
Buffer PBS, 0.02 % Sodium Azide, 0.1 % BSA
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 2-8 °C.
Allgemeine Veröffentlichungen Pelletier, Okawara, Vitale, Anderson: "Differential distribution of the tight-junction-associated protein ZO-1 isoforms alpha+ and alpha- in guinea pig Sertoli cells: a possible association with F-actin and G-actin." in: Biology of reproduction, Vol. 57, Issue 2, pp. 367-76, 1997 (PubMed).

Van Itallie, Balda, Anderson: "Epidermal growth factor induces tyrosine phosphorylation and reorganization of the tight junction protein ZO-1 in A431 cells." in: Journal of cell science, Vol. 108 ( Pt 4), pp. 1735-42, 1995 (PubMed).

Balda, Anderson: "Two classes of tight junctions are revealed by ZO-1 isoforms." in: The American journal of physiology, Vol. 264, Issue 4 Pt 1, pp. C918-24, 1993 (PubMed).

Willott, Balda, Heintzelman, Jameson, Anderson: "Localization and differential expression of two isoforms of the tight junction protein ZO-1." in: The American journal of physiology, Vol. 262, Issue 5 Pt 1, pp. C1119-24, 1992 (PubMed).

Kurihara, Anderson, Farquhar: "Diversity among tight junctions in rat kidney: glomerular slit diaphragms and endothelial junctions express only one isoform of the tight junction protein ZO-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 15, pp. 7075-9, 1992 (PubMed).