Myc Tag Antikörper (AA 410-419) (Biotin)

Produktnummer ABIN1103150

Produktdetails anti-Myc Tag Antikörper (Biotin)

Target: Myc Tag

Bindungsspezifität: AA 410-419

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Myc Tag Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Spezifität: This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum.

Aufreinigung: Affinity purified

Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.

Type of Immunogen: Purified protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: Anti-myc has utility to detect the fusion protein of the myc epitope cloned along with the target gene. As such, anti-myc/myc can be used to identify fusion proteins containing the myc epitope. The antibody recognizes the Myc tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and myc containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. Biotin conjugated anti-EPITOPE tag antibodies are generally suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA, 0.01 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Details zu Myc Tag

Target: Myc Tag

Abstract: Myc Tag Produkte

Substanzklasse: Tag