ATP7A Antikörper (C-Term)

Produktnummer ABIN1098198

Produktdetails anti-ATP7A Antikörper

Target: ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Bindungsspezifität: AA 42-61, C-Term

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ATP7A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Verwendungszweck: Anti-Copper-Transporting ATPase1 Mouse Monoclonal Antibody

Spezifität: This antibody recognizes human, mouse, and rat Copper-Transporting ATPase1.Immunohistochemistry: use at 1-5 μg/mL. These are recommended concentrations.Enduser should determine optimal concentrations for their

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein G affinity chromatography.

Immunogen: Synthetic peptide correspond- ing to aa 42-61 (cytoplasmic C-terminus) of human Copper-Transporting ATPase1 (accession no. NP_000043.3).

Klon: S60-4

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunoblotting: use at 1-2 μg/mL. A band of ~180 kDa is detected.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Details zu ATP7A

Target: ATP7A (ATPase, Cu++ Transporting, alpha Polypeptide (ATP7A))

Andere Bezeichnung: Copper-Transporting ATPase1 (ATP7A Produkte)

Synonyme: ATP7A antikoerper, cal antikoerper, wu:fc43e01 antikoerper, zgc:153422 antikoerper, zgc:158633 antikoerper, DDBDRAFT_0218568 antikoerper, DDBDRAFT_0235190 antikoerper, DDB_0218568 antikoerper, DDB_0235190 antikoerper, atpase antikoerper, Atp7a antikoerper, kal antikoerper, atp7a antikoerper, DSMAX antikoerper, MK antikoerper, MNK antikoerper, SMAX3 antikoerper, Blo antikoerper, DXHXS1608e antikoerper, I14 antikoerper, Mo antikoerper, blotchy antikoerper, br antikoerper, brindled antikoerper, mottled antikoerper, Mnk antikoerper, ATPase copper transporting alpha antikoerper, ATPase, Cu++ transporting, alpha polypeptide antikoerper, P-type ATPase antikoerper, ATP synthase subunit a antikoerper, copper-transporting ATPase 1 antikoerper, ATP7A antikoerper, atp7a antikoerper, LOC100049514 antikoerper, Atp7a antikoerper, LOC412379 antikoerper

Hintergrund: Copper-transporting ATPase 1,ATP-driven copper (Cu(+)) ion pump that plays an important role in intracellular copper ion homeostasis (PubMed:10419525, PubMed:11092760, PubMed:28389643). Within a catalytic cycle, acquires Cu(+) ion from donor protein on the cytoplasmic side of the membrane and delivers it to acceptor protein on the lumenal side. The transfer of Cu(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state (PubMed:10419525, PubMed:19453293, PubMed:19917612, PubMed:31283225, PubMed:28389643). Under physiological conditions, at low cytosolic copper concentration, it is localized at the trans-Golgi network (TGN) where it transfers Cu(+) ions to cuproenzymes of the secretory pathway (PubMed:28389643, PubMed:11092760). Upon elevated cytosolic copper concentrations, it relocalizes to the plasma membrane where it is responsible for the export of excess Cu(+) ions (PubMed:10419525, PubMed:28389643). May play a dual role in neuron function and survival by regulating cooper efflux and neuronal transmission at the synapse as well as by supplying Cu(+) ions to enzymes such as PAM, TYR and SOD3 (PubMed:28389643) (By similarity). In the melanosomes of pigmented cells, provides copper cofactor to TYR to form an active TYR holoenzyme for melanin biosynthesis (By similarity). {UniProtKB:Q64430, PubMed:10419525, PubMed:11092760, PubMed:19453293, PubMed:19917612, PubMed:28389643, PubMed:31283225}.,The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from cells. ATP7A (also known as Copper-Transporting ATPase1) functions as a transmembrane copper-translocating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Although ATP7A is not detectable in most normal tissues, it is expressed in many common tumor types. Increased expression of ATP7A renders tumor cells resistant to cisplatin and carboplatin.,Golgi apparatus, trans-Golgi network membrane, Cell membrane, Melanosome membrane, Early endosome membrane, Cell projection, axon, Cell projection, dendrite, Cell junction, synapse, postsynaptic density,EC 7.2.2.8, Copper pump 1, Menkes disease-associated protein

UniProt: Q04656

Pathways: Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process