Tumor Protein P53 (TP53) Antikörper

Antigen: Tumor Protein P53 (TP53)

Synonyme: p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53

Klonalität: Monoklonal (BP53-12)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus), Schaf

Applikation: Immunhistochemie (IHC), Immunzytochemie (ICC), Chromatin-Immunopräzipitation (ChiP), ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN105263

Produktbeschreibung

Weitere Bezeichnung: p53

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p53 protein. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma P3- X63/AG8.653 cells using conventional hybridoma technology.

Format: Liquid (sterile filtered)

Isotyp: IgG2a kappa  (Passende Sekundärantikörper)

Klon: BP53-12

Beschreibung: The p53 gene like the Rb gene, is a tumor suppressor gene, i.e., its activity stops the formation of tumors. If a person inherits only one functional copy of the p53 gene from their parents, they are predisposed to cancer and usually develop several independent tumors in a variety of tissues in early adulthood. This condition is rare, and is known as Li-Fraumeni syndrome. However, mutations in p53 are found in most tumor types, and so contribute to the complex network of molecular events leading to tumor formation. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). The p53 gene has been mapped to chromosome 17. In the cell, p53 protein binds DNA, which in turn stimulates another gene to produce a protein called p21 that interacts with a cell division-stimulating protein (cdk2). When p21 is complexed with cdk2 the cell cannot pass through to the next stage of cell division. Mutant p53 can no longer bind DNA in an effective way, and as a consequence the p21 protein is not made available to act as the 'stop signal' for cell division. Thus cells divide uncontrollably, and form tumors. p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Help with unraveling the molecular mechanisms of cancerous growth has come from the use of mice as models for human cancer, in which powerful 'gene knockout' techniques can be used. The amount of information that exists on all aspects of p53 normal function and mutant expression in human cancers is now vast, reflecting its key role in the pathogenesis of human cancers. It is clear that p53 is just one component of a network of events that culminate in tumor formation.

Spezifität: This protein A purified mouse monoclonal antibody reacts specifically with p53 in human tissues and cell lines. The antibody recognizes a 53 kDa band corresponding to p53. Cross reactivity with p53 from other sources has not been determined.

Anwendungen

Applikationshinweise: Recommended Dilutions: This product was assayed by immunoblot using HRP Goat-anti-Mouse IgG and ECL for detection. A working dilution of 1:500 to 1:1,000 is suggested for this assay. For immunohistochemistry dilute the antibody 1:50. Researchers should determine optimal titers for other applications. Note: This antibody is suitable for flow cytometry, immunohistochemistry, immunoblotting and immunoprecipitation. p53 is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dental fibroblasts following mild ultraviolet irradiation. This antibody reacts with a N-terminal epitope of the 53 kD gene product and this epitope is not destroyed by formalin-fixation and routine paraffin embedding. Microwaving is needed for optimal staining.

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Forschungsgebiet: Krebs, Zellzyklus, Transkriptionsfaktoren, DNS/RNS, Apoptose/Nekrose

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blotting using anti-p53. HeLa whole cell lysate (lane 1), cytosol fraction (lane 2) and nuclear extract (lane 3) (15 ug) were separated by 10% SDS-PAGE and transferred to nitrocellulose membrane. The membrane was blocked with 3% milk/TBST for 1 h at room temperature followed by incubation with antibodies-online's Protein A purified Mab anti-povernight at 4

Immunofluorescence microscopy of Hcells using anti-p53. antibodies-online's Protein A purifiedMab anti-p53 was used at a 1:100 dilution in 10% normal goat serum in PBS and reacted overni4