HA-Tag Antikörper

Details zu Produkt Nr. ABIN1043870, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
444
33
20
2
1
1
1
1
Wirt
Kaninchen
205
180
40
34
20
3
Klonalität
Polyklonal
Konjugat
Unkonjugiert
45
31
24
10
10
9
9
8
7
7
7
7
6
6
6
6
6
6
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
390
201
122
117
85
82
70
40
15
13
11
7
7
5
4
4
4
4
3
2
2
2
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
Immunogen Type: Peptide
Sequenz YPYDVPDYA
Isotyp IgG
Spezifität This affinity purified Anti-HA antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date, this antibody has reacted with all HA-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Produktmerkmale Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of
Sterilität Sterile filtered
Andere Bezeichnung HA tag
Substanzklasse Tag
Hintergrund Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of
Forschungsgebiet Tags/Labels
Applikationshinweise Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. The antibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targeted proteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and HA containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.95 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Haltbarkeit 12 months
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-HA-Tag antibody (ABIN1043870) Affinity Purified anti-HA epitope tag polyclonal antibody detects HA tagged recombina...
 image for anti-HA-Tag antibody (ABIN1043870) anti-HA-Tag antibody (Image 2)
Allgemeine Veröffentlichungen Field, Nikawa, Broek, MacDonald, Rodgers, Wilson, Lerner, Wigler: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).

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