IGF1 Protein

Produktnummer ABIN935504

Produktdetails

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Produktmerkmale: Purified recombinant Human IGF1 protein
Expression System: E.coli
Bioactivity: The ED50, calculated by the dose-dependant proliferation of murine BALB\\C 3T3 cells (measured by 3H-thymidine uptake) is

Reinheit: > 98 % pure

Anwendungsinformationen

Applikationshinweise: Each Investigator should determine their own optimal working dilution for specific applications.

Testdurchführung:

Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in distiled water to a concentration of 0.1-1.0 mg/mL.

Buffer: Supplied as a lyophilized powder.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: RT/-20 °C

Antigendetails

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Andere Bezeichnung: IGF1 (IGF1 Produkte)

Hintergrund: The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH, MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2, MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986, Rosenfeld, 2003).
Alternative Names: IGF-IA protein, IGF1 protein, Somatomedin C protein, MGF protein, IGF-1 protein, IGF-1, IGF-I protein, Mechano growth factor protein, IGF 1, Insulin-Like Growth Factor 1 protein, IGFI protein, IGF1, IGF 1 protein

Molekulargewicht: 7.655 kDa

Pathways: RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation