EGF Protein

Produktnummer ABIN935319

Dieses Recombinant EGF-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für EGF Protein (ABIN935319)

Target: EGF (Epidermal Growth Factor (EGF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 98 % pure

Produktdetails

Produktmerkmale: Purified recombinant Human EGF protein
Expression System: E.coli
Bioactivity: The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is

Anwendungsinformationen

Applikationshinweise: Each Investigator should determine their own optimal working dilution for specific applications.

Testdurchführung:

EGF quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of
2.858 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lypholized rHuEGF in sterile water not less than 100 µg/mL.

Buffer: Supplied as a lyophilized powder.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: RT/-20 °C

Antigendetails

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF

Hintergrund: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
Alternative Names: Epidermal Growth Factor protein, Urogastrone protein, URG protein

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation