EGF Protein

Produktnummer ABIN934920

Dieses Recombinant EGF-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für EGF Protein (ABIN934920)

Target: EGF (Epidermal Growth Factor (EGF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 95 % pure

Produktdetails

Sequenz: MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR

Produktmerkmale: Purified recombinant Human EGF protein
Expression System: E.coli
Bioactivity: The ED50 for this effect is < 0.89 ng/mL . Measured in a cell proliferation assay using mouse Balb3T3 cell.
Molecular weight on SDS-PAGE will appear higher.

Anwendungsinformationen

Applikationshinweise: EGF protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Testdurchführung:

Cell line: Balb-3T3 (Mus musculus (mouse)) Maintenance Condition: RPMI 1640 containing 10 % FBS Assay medium: serum free RPMI 1640 Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100 µL) Serum Free Starvation: 24 h with RPMI1640 Incubation time: 24 hr (after sample treatment) Concentration range: 1 pg/mL - 1 µg/mL Detection method: BrdU assay

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Supplied as a liquid in PBS, pH 7.4.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: RT/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Antigendetails

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF

Hintergrund: Recombinant human epidermal growth factor(EGF) is a 6.3 kDa globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Alternative Names: Epidermal growth factor protein, Urogastrone protein, Epidermal growth factor protein, epidermal growth factor (beta-urogastrone), EGF protein, Pro-epidermal growth factor protein

Molekulargewicht: 6.3 kDa (54 AA)

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation