c-MYC Protein (AA 1-439, full length)

Produktnummer ABIN8117620

Recombinant c-MYC-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für c-MYC Protein (AA 1-439, full length) (ABIN8117620)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: Greater than 85 % as determined by SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 1-439, full length

Verwendungszweck: Recombinant Human MYC protein

Sequenz: MPLNVSFTNR NYDLDYDSVQ PYFYCDEEEN FYQQQQQSEL QPPAPSEDIW KKFELLPTPP LSPSRRSGLC SPSYVAVTPF SLRGDNDGGG GSFSTADQLE MVTELLGGDM VNQSFICDPD DETFIKNIII QDCMWSGFSA AAKLVSEKLA SYQAARKDSG SPNPARGHSV CSTSSLYLQD LSAAASECID PSVVFPYPLN DSSSPKSCAS QDSSAFSPSS DSLLSSTESS PQGSPEPLVL HEETPPTTSS DSEEEQEDEE EIDVVSVEKR QAPGKRSESG SPSAGGHSKP PHSPLVLKRC HVSTHQHNYA APPSTRKDYP AAKRVKLDSV RVLRQISNNR KCTSPRSSDT EENVKRRTHN VLERQRRNEL KRSFFALRDQ IPELENNEKA PKVVILKKAT AYILSVQAEE QKLISEEDLL RKRREQLKHK LEQLRNSCA

Anwendungsinformationen

Kommentare:

Tag-Free

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid, Lyophilized

Rekonstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50 % of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50 %. Customers could use it as reference.

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5 % -50 % glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6 % Trehalose, pH 8.0.

Handhabung: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Haltbarkeit: 6-12 months

Antigendetails

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Andere Bezeichnung: MYC

Hintergrund:

Relevance: Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis. Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells. Functions with TAF6L to activate target gene expression through RNA polymerase II pause release. Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform

Alternative Name(s): Class E basic helix-loop-helix protein 39,Proto-oncogene c-Myc,Transcription factor p64

Molekulargewicht: 48.9 kDa

UniProt: P01106

Pathways: p53 Signalweg, Zellzyklus, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effekt