p21 Protein

Produktnummer ABIN7938737

Dieses Recombinant p21-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für p21 Protein (ABIN7938737)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Produktdetails

Verwendungszweck: Recombinant CDKN1A protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Recombinant CDKN1A protein is in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 5 % glycerol.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Andere Bezeichnung: CDKN1A

Hintergrund:

Protein Background: CDKN1A (Cyclin-dependent kinase inhibitor 1) may be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding.Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest.

Short Description: Recombinant CDKN1A protein that includes amino acids 1-164 of human CDKN1A protein (accession number NP_000380.1) was expressed was expressed in E. coli cells with an N-terminal 6x His tag. The molecular weight of the protein is 20.05 kDa.

Aliases: Cyclin-dependent kinase inhibitor 1, also known as P21, CIP1, CDK1A, P21CIP1

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagie