TLR3 Protein (AA 24-904)

Produktnummer ABIN7866071

Recombinant TLR3-Protein exprimiert in CHO Cells.

Kurzübersicht für TLR3 Protein (AA 24-904) (ABIN7866071)

Target: TLR3 (Toll-Like Receptor 3 (TLR3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: CHO Cells

Reinheit: >95 % , as determined by SDS-PAGE and HPLC

Produktdetails

Proteineigenschaft: AA 24-904

Verwendungszweck: Human TLR3 Protein Recombinant

Sequenz: STTKCTVSHE VADCSHLKLT QVPDDLPTNI TVLNLTHNQL RRLPAANFTR YSQLTSLDVG FNTISKLEPE LCQKLPMLKV LNLQHNELSQ LSDKTFAFCT NLTELHLMSN SIQKIKNNPF VKQKNLITLD LSHNGLSSTK LGTQVQLENL QELLLSNNKI QALKSEELDI FANSSLKKLE LSSNQIKEFS PGCFHAIGRL FGLFLNNVQL GPSLTEKLCL ELANTSIRNL SLSNSQLSTT SNTTFLGLKW TNLTMLDLSY NNLNVVGNDS FAWLPQLEYF FLEYNNIQHL FSHSLHGLFN VRYLNLKRSF TKQSISLASL PKIDDFSFQW LKCLEHLNME DNDIPGIKSN MFTGLINLKY LSLSNSFTSL RTLTNETFVS LAHSPLHILN LTKNKISKIE SDAFSWLGHL EVLDLGLNEI GQELTGQEWR GLENIFEIYL SYNKYLQLTR NSFALVPSLQ RLMLRRVALK NVDSSPSPFQ PLRNLTILDL SNNNIANIND DMLEGLEKLE ILDLQHNNLA RLWKHANPGG PIYFLKGLSH LHILNLESNG FDEIPVEVFK DLFELKIIDL GLNNLNTLPA SVFNNQVSLK SLNLQKNLIT SVEKKVFGPA FRNLTELDMR FNPFDCTCES IAWFVNWINE THTNIPELSS HYLCNTPPHY HGFPVRLFDT SSCKDSAPFE L

Produktmerkmale:

Protein Size: Recombinant TLR3 migrates as an approximately 75 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding Human TLR3 mature chain was expressed in CHO. Recombinant Human Toll-like receptor 3 encodes amino acid residues 24-904.

Endotoxin-Niveau: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: Determined by its ability to neutralize Poly I:C induced NF-κB signaling in HT-29 cells stably transfected with NF-κB-luc. The ED50 for this effect is 5-10 μg/ml.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: TLR3 was Lyophilized from PBS, pH 7.2.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 24 months

Antigendetails

Target: TLR3 (Toll-Like Receptor 3 (TLR3))

Andere Bezeichnung: TLR3

Molekulargewicht: 75 kDa

Pathways: TLR Signalweg, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades