EGF Protein

Produktnummer ABIN7680472

Recombinant EGF-Protein exprimiert in Yeast (Saccharomyces Cerevisiae).

Kurzübersicht für EGF Protein (ABIN7680472)

Target: EGF (Epidermal Growth Factor (EGF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Yeast (Saccharomyces Cerevisiae)

Applikation: Cell Culture (CC)

Reinheit: More than 95 % as determined by SDS-PAGE. 

Güteklasse: Animal-Free

Produktdetails

Verwendungszweck: rhEGF - Recombinant human Epidermal growth factor

Produktmerkmale: This product arrives as White lyophilized powder 

Endotoxin-Niveau: Less than 1 EU/mg

Biological Activity Comment: The ED50 is ≤1ng/ml determined by a cell proliferation assay using Balb/c 3T3 cell, corresponding to a specific activity of ≥1x10^6 Units/mg.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is recommended to reconstitute the lyophilized OsrhEGF in sterile water.

Buffer: It is lyophilized with Mannitol as the stabilizer.

Handhabung: Use as soon as possible after opening. Avoid freeze-thaw cycles

Lagerung: -20 °C

Informationen zur Lagerung: Stored at temperatures -20°C for at least 36 months.  Use as soon as possible after opening. Please avoid freeze-thaw cycles.

Haltbarkeit: 36 months

Antigendetails

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: Epidermal growth factor

Hintergrund: Human epidermal growth factor, also known as EGF, is a 6.2kD protein with 53 amino acid residues and three intramolecular disulfide bonds. By binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface, EGF results in a variety of biochemical changes within the cell, rising intracellular calcium levels, increasing glycolysis, protein synthesis and regulating the gene expression, including the gene for EGFR that ultimately lead to DNA synthesis and cell proliferation.

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation