IL1A Protein (AA 113-271)

Produktnummer ABIN7566200

Dieses Recombinant IL1A-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für IL1A Protein (AA 113-271) (ABIN7566200)

Target: IL1A (Interleukin 1 alpha (IL1A))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: >95 % (SDS-PAGE)

Produktdetails

Proteineigenschaft: AA 113-271

Verwendungszweck: IL-1alpha (human) (rec.)

Kreuzreaktivität: Human

Produktmerkmale: Human IL-1alpha (aa 113-271).

Sterilität: Sterile filtered

Endotoxin-Niveau: <0.01EU/μg protein (LAL test, Lonza).

Biological Activity Comment: The ED50 as determined by the dose-dependent stimulation of murine D10S cells is < 0.001 ng/ml, corresponding to a specific activity of > 1 x 109units/mg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handhabung: Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1 % BSA should be used for further dilutions.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Antigendetails

Target: IL1A (Interleukin 1 alpha (IL1A))

Andere Bezeichnung: IL-1alpha

Hintergrund:

Interleukin-1alpha, IL-1F1, IL-1A

The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent manner.

Molekulargewicht: ~19kDa (SDS-PAGE)

NCBI Accession: NP_000566

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints