EPO Protein (AA 28-193)

Produktnummer ABIN7504320

Dieses Recombinant EPO-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für EPO Protein (AA 28-193) (ABIN7504320)

Target: EPO (Erythropoietin (EPO))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 28-193

Verwendungszweck: Human EPO/Erythropoietin Protein

Sequenz: Ala28-Arg193

Produktmerkmale: Recombinant Human EPO/Erythropoietin Protein is expressed from HEK293 without tag.It contains Ala28-Arg193.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 0.1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human EPO, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 46.1ng/ml determined by ELISA.,Human EPO on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50-for this effect is 0.4 - 1 ng/mL (QC Test).,The purity of Human EPO is greater than 95% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: EPO (Erythropoietin (EPO))

Andere Bezeichnung: EPO

Substanzklasse: Hormone

Hintergrund: Erythropoietin (EPO) is a circulating hormone conventionally considered to be responsible for erythropoiesis. In addition to facilitating red blood cell production, EPO has pluripotent potential, such as for cognition improvement, neurogenesis, and anti-fibrotic, anti-apoptotic, anti-oxidative, and anti-inflammatory effects.

Molekulargewicht: 18.39 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.

Pathways: JAK-STAT Signalweg, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity