IL-3 Protein (AA 20-152)

Produktnummer ABIN7454153

Dieses Recombinant IL-3-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für IL-3 Protein (AA 20-152) (ABIN7454153)

Target: IL-3 (Interleukin 3 (IL-3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 20-152

Verwendungszweck: Human IL-3 Protein

Sequenz: Ala20-Phe152

Produktmerkmale: Recombinant Human IL-3 Protein is expressed from E.coli without tag.It contains Ala20-Phe152.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 0.05 EU per μg by the LAL method.

Biological Activity Comment: Human IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50-for this effect is 0.15 - 0.75 ng/mL (QC Test).,The purity of Human IL-3 is greater than 95% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IL-3 (Interleukin 3 (IL-3))

Andere Bezeichnung: IL-3

Hintergrund: Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Molekulargewicht: 15.09 kDa same as Bis-Tris PAGE result.

Pathways: JAK-STAT Signalweg, Regulation of Carbohydrate Metabolic Process, Autophagie