VEGFR2/CD309 Protein (AA 20-764) (mFc Tag,Biotin)

Produktnummer ABIN7275834

Produktdetails

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 20-764

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses VEGFR2/CD309 Protein ist gelabelt mit mFc Tag,Biotin.

Verwendungszweck: Biotinylated Human VEGF R2/KDR Protein (Primary Amine Labeling)

Sequenz: Ala20-Glu764

Produktmerkmale: Recombinant Biotinylated Human VEGF R2/KDR Protein (Primary Amine Labeling) is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ala20-Glu764.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human VEGF165, No Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human VEGF R2, mFc Tag with the EC50 of 0.14μg/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Andere Bezeichnung: VEGF R2 (VEGFR2 Produkte)

Hintergrund: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. The tyrosine kinase receptor vascular endothelial growth factor receptor 2 (VEGFR2) is a key regulator of angiogenesis.

Molekulargewicht: 110 kDa. Due to glycosylation, the protein migrates to 135-160 kDa based on Tris-Bis PAGE result.

UniProt: P35968-1

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling