LRP6 Protein (AA 20-630) (mFc Tag)

Produktnummer ABIN7275210

Dieses Recombinant LRP6-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für LRP6 Protein (AA 20-630) (mFc Tag) (ABIN7275210)

Target: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 90 % as determined by Tris-Bis PAGE

Produktdetails

Proteineigenschaft: AA 20-630

Aufreinigungstag / Konjugat: Dieses LRP6 Protein ist gelabelt mit mFc Tag.

Verwendungszweck: Human LRP-6 Protein

Sequenz: Ala20-Pro630

Produktmerkmale: Recombinant Human LRP-6 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ala20-Pro630.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human DKK1, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 2.1μg/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Andere Bezeichnung: LRP-6

Hintergrund: Dickkopf-related protein 1 (DKK-1), a ligand for the WNT coreceptors low-density lipoprotein receptor-related proteins 5 and 6 (LRP-5 and LRP-6) and an inhibitor of WNT/β-catenin signaling, effectively inhibits pericyte activation, detachment, and transition to myofibroblasts in vivo in response to kidney injury, resulting in attenuated fibrogenesis, capillary rarefaction, and inflammation.LRP-6 interacts closely with PDGF receptor β and TGF-β receptor 1 at the cell membrane, suggesting that it may have roles in pathways other than WNT/β-catenin.

Molekulargewicht: 95.3 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result.

UniProt: O75581-1

Pathways: WNT Signalweg, Tube Formation