IL1A Protein (AA 113-271)

Produktnummer ABIN7274879

Recombinant IL1A-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für IL1A Protein (AA 113-271) (ABIN7274879)

Target: IL1A (Interleukin 1 alpha (IL1A))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 113-271

Verwendungszweck: Human IL-1 alpha/IL-1A Protein

Sequenz: Ser113-Ala271

Produktmerkmale: Recombinant Human IL-1 alpha/IL-1A Protein is expressed from E.coli without tag.It contains Ser113-Ala271.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 0.05 EU per μg by the LAL method.

Biological Activity Comment: Human IL-1R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-1 alpha, No tag with an affinity constant of 0.51 nM as determined in SPR assay (Biacore T200).,Human IL-1 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human IL-1 alpha is greater than 95% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IL1A (Interleukin 1 alpha (IL1A))

Andere Bezeichnung: IL-1 alpha

Hintergrund: The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases.

Molekulargewicht: 18 kDa same as Bis-Tris PAGE result.

NCBI Accession: NP_000566

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints