ErbB2/Her2 Protein (AA 23-652) (His tag)

Produktnummer ABIN7274794

Produktdetails

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 23-652

Spezies: Cynomolgus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses ErbB2/Her2 Protein ist gelabelt mit His tag.

Verwendungszweck: Cynomolgus Her2/ErbB2 Protein

Sequenz: Thr23-Thr652

Produktmerkmale: Recombinant Cynomolgus Her2/ErbB2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr23-Thr652.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus Her2, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Andere Bezeichnung: Her2 (ErbB2/Her2 Produkte)

Hintergrund: ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane.

Molekulargewicht: 70.4 kDa. Due to glycosylation, the protein migrates to 85-105 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_005584091

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Skeletal Muscle Fiber Development