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EGF Protein (AA 971-1023)

EGF Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Produktnummer ABIN7274522
  • Target Alle EGF Proteine anzeigen
    EGF (Epidermal Growth Factor (EGF))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 971-1023
    Spezies
    • 26
    • 16
    • 9
    • 4
    • 3
    • 1
    Human
    Quelle
    • 38
    • 10
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Verwendungszweck
    Human EGF Protein
    Sequenz
    Asn971-Arg1023
    Produktmerkmale
    Recombinant Human EGF Protein is expressed from E.coli without tag.It contains Asn971-Arg1023.
    Reinheit
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Less than 0.2 EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human EGF at 2μg/ml (100μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 81.0ng/ml determined by ELISA. The affinity constant of 0.05 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Lagerung
    -20 °C,-80 °C
    Informationen zur Lagerung
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    EGF (Epidermal Growth Factor (EGF))
    Andere Bezeichnung
    EGF (EGF Produkte)
    Hintergrund
    The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
    Molekulargewicht
    5.6 kDa same as Tris-Bis PAGE result.
    UniProt
    P01133-1
    Pathways
    NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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