EGF Protein (AA 971-1023)
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- Target Alle EGF Proteine anzeigen
- EGF (Epidermal Growth Factor (EGF))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 971-1023
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Verwendungszweck
- Human EGF Protein
- Sequenz
- Asn971-Arg1023
- Produktmerkmale
- Recombinant Human EGF Protein is expressed from E.coli without tag.It contains Asn971-Arg1023.
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 0.2 EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human EGF at 2μg/ml (100μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 81.0ng/ml determined by ELISA. The affinity constant of 0.05 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- EGF (Epidermal Growth Factor (EGF))
- Andere Bezeichnung
- EGF (EGF Produkte)
- Hintergrund
- The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
- Molekulargewicht
- 5.6 kDa same as Tris-Bis PAGE result.
- UniProt
- P01133-1
- Pathways
- NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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