DLL4 Protein (AA 27-524) (Fc Tag)

Produktnummer ABIN7274485

Produktdetails

Target: DLL4 (delta-Like 4 (DLL4))

Protein-Typ: Recombinant

Proteineigenschaft: AA 27-524

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses DLL4 Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Human DLL4 Protein

Sequenz: Ser27-Pro524

Produktmerkmale: Recombinant Human DLL4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser27-Pro524.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human DLL4, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-DLL4 Antibody, hFc Tag with the EC50 of 17.8ng/ml determined by ELISA. The affinity constant of 0.48 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: DLL4 (delta-Like 4 (DLL4))

Andere Bezeichnung: DLL4 (DLL4 Produkte)

Hintergrund: Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis, negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.

Molekulargewicht: 81.1 kDa. Due to glycosylation, the protein migrates to 82-90 kDa based on Tris-Bis PAGE result.

UniProt: Q9NR61

Pathways: Notch Signalweg