CSF1R Protein (AA 20-512) (His-Avi Tag)

Produktnummer ABIN7274427

Dieses Recombinant CSF1R-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für CSF1R Protein (AA 20-512) (His-Avi Tag) (ABIN7274427)

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 20-512

Aufreinigungstag / Konjugat: Dieses CSF1R Protein ist gelabelt mit His-Avi Tag.

Verwendungszweck: Human M-CSF R/CSF1R/CD115 Protein

Sequenz: Ile20-Glu512

Produktmerkmale: Recombinant Human M-CSF R/CSF1R/CD115 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile20-Glu512.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human M-CSF R, His Tag at 1μg/ml (100μl/Well). Dose response curve for Anti-M-CSF R Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Andere Bezeichnung: M-CSF R

Hintergrund: CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.

Molekulargewicht: 57.4 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.

UniProt: P07333-1

Pathways: RTK Signalweg, Inositol Metabolic Process, Cell-Cell Junction Organization