PDK4 Protein (AA 1-412) (His-GST)

Produktnummer ABIN7320000

Dieses Recombinant PDK4-Protein wird in Baculovirus infected Insect Cells produziert.

Kurzübersicht für PDK4 Protein (AA 1-412) (His-GST) (ABIN7320000)

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Baculovirus infected Insect Cells

Reinheit: > 85 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 1-412

Aufreinigungstag / Konjugat: Dieses PDK4 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Mouse PDK4 Protein (His & GST Tag)

Sequenz: Met1-Val412

Produktmerkmale: A DNA sequence encoding the mouse PDK4 (O70571) (Met1-Val412) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Kinase activity untested

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Andere Bezeichnung: PDK4

Hintergrund: AV005916,Pyruvate dehydrogenase kinase 4 (PDK4) is a mitochondrial protein that regulates the TCA cycle.PDK4, a vital mitochondrial protein, controls the switch between glycolysis and oxidative phosphorylation based upon nutrient availability.Pyruvate dehydrogenase kinase 4 (PDK4) mRNA has been reported as an up-regulated gene in the heart and skeletal muscle of carnitine-deficient juvenile visceral steatosis (JVS) mice under fed conditions. PDK4 plays an important role in the inhibition of glucose oxidation via the phosphorylation of pyruvate dehydrogenase complex (PDC).PDK4 gene expression is stimulated by thyroid hormone (T(3)), glucocorticoids, and long chain fatty acids.

Molekulargewicht:

Calculated MW: 74.4 kDa

Observed MW: 65 kDa

UniProt: O70571

Pathways: PI3K-Akt Signalweg, Hedgehog Signalweg, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effekt