LIF Protein

Produktnummer ABIN7565330

Dieses Recombinant LIF-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für LIF Protein (ABIN7565330)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Cell Culture (CC)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Verwendungszweck: Recombinant Human LIF Protein (HEK293 Cells)

Sequenz: Met 1-Phe202

Produktmerkmale: A DNA sequence encoding the human LIF (P15018) (Met1-Phe202) was expressed.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells. The ED₅₀ for this effect is typically 0.2-0.8 ng/mL.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: LIF (Leukemia Inhibitory Factor (LIF))

Andere Bezeichnung: LIF

Hintergrund: CDF;D Factor;DIA;Differentiation-Stimulating Factor;Emfilermin;HILDA;LIF;Leukemia Inhibitory Factor;MLPLI;Melanoma-Derived LPL Inhibitor,Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It’s involved in growth promotion and cell differentiation of different types of target cells; influence on bone metabolism; cachexia; neural development; embryogenesis and inflammation. LIF has potent proinflammatory property; being the inducer of the acute phase protein synthesis and affecting the cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells; hematopoietic and neuronal cells. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors; the GP130 signal transducing subunit. This leads to activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities; LIF is involved in the pathogenic events and development of many diseases of various origin.

Molekulargewicht:

Calculated MW: 19.7 kDa

Observed MW: 35.4 kDa

Gen-ID: 3976

UniProt: P15018

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding