LIF Protein (AA 23-202)

Produktnummer ABIN2018195

Produktdetails

Target: LIF (Leukemia Inhibitory Factor (LIF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 23-202

Spezies: Human

Quelle: Escherichia coli (E. coli)

Produktmerkmale: ED50 < 0.2 ng/mL, measured by a cell differentiation assay using TF-I cells, corresponding to a specific activity of > 5 x 10^6 units/mg.

Reinheit: > 95 % by SDS-PAGE analysis.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant human Leukemia Inhibitory Factor (rhLIF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhLIF remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C. ,

Haltbarkeit: 6 months

Referenzen

Huang, Li, Alonso-Gonzalez, Gorre, Keatley, Green, Turner, Kallingappa, Verma, Oback: "A virus-free poly-promoter vector induces pluripotency in quiescent bovine cells under chemically defined conditions of dual kinase inhibition." in: PLoS ONE, Vol. 6, Issue 9, pp. e24501, (2011) (PubMed).

Yu, Kastin, Pan: "TNF reduces LIF endocytosis despite increasing NFkappaB-mediated gp130 expression." in: Journal of cellular physiology, Vol. 213, Issue 1, pp. 161-6, (2007) (PubMed).

Antigendetails

Target: LIF (Leukemia Inhibitory Factor (LIF))

Abstract: LIF Produkte

Synonyme: CDF Protein, DIA Protein, HILDA Protein, MLPLI Protein, LIF, interleukin 6 family cytokine Protein, leukemia inhibitory factor Protein, LIF Protein, Lif Protein

Hintergrund: Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine belonging to the long four-helix bundle cytokine superfamily. LIF shares tertiary structure with several other cytokines, including Interleukin-6 (IL-6), Oncostatin M, ciliary neurotropic factor, and cardiotrophin-1, and their functions in vivo are also redundant to some extent. LIF can bind to the common receptor of IL-6 subfamily, gp130, and then recruit its own receptor LIF Receptor to form a ternary complex. The basal expression of LIF in vivo is low, and its expression is induced by pro-inflammatory factors, including lipopolysaccharide, IL-1, and IL-17, and inhibited by anti-inflammatory agents, including IL-4 and IL-13. The functions of LIF include proliferation of primordial germ cells, regulation in blastocyst implantation and early pregnancy, and maintenance of pluripotent embryonic stem cells.Recombinant human Leukemia Inhibitory Factor (rhLIF) produced in E. coli is a single non-glycosylated polypeptide chain containing 180 amino acids. A fully biologically active molecule, rhLIF has a molecular mass of 19.7 kDa analyzed by reducing SDS-PAGE.
Synonyms: D factor, MLPLI

Molekulargewicht: 19.7 kDa, observed by reducing SDS-PAGE.

UniProt: P15018

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding