CDK1 Protein (AA 1-297) (His-GST)

Produktnummer ABIN7195307

Recombinant CDK1-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für CDK1 Protein (AA 1-297) (His-GST) (ABIN7195307)

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Baculovirus infected Insect Cells

Reinheit: > 85 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 1-297

Aufreinigungstag / Konjugat: Dieses CDK1 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Mouse CDC2 Kinase/CDK1 Protein (His & GST Tag)

Sequenz: Met1-Met297

Produktmerkmale: A DNA sequence encoding the mouse CDK1 (P11440) (Met1-Met297) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Kinase activity untested

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM Nacl, 10 % glycerol, pH 8.0

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Andere Bezeichnung: CDC2 Kinase/CDK1

Hintergrund: CDC2>Cdc2;Cdc2a;p34<,CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.

Molekulargewicht:

Calculated MW: 61.9 kDa

Observed MW: 57 kDa

UniProt: P11440

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA