CHEK1 Protein (AA 1-476) (His-GST)

Produktnummer ABIN7320012

Dieses Recombinant CHEK1-Protein wird in Baculovirus infected Insect Cells exprimiert.

Kurzübersicht für CHEK1 Protein (AA 1-476) (His-GST) (ABIN7320012)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Baculovirus infected Insect Cells

Reinheit: > 90 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 1-476

Aufreinigungstag / Konjugat: Dieses CHEK1 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Mouse CHK1/CHEK1 Protein (His & GST Tag)

Sequenz: Met1-Thr476

Produktmerkmale: A DNA sequence encoding the mouse CHEK1 (O35280-1) (Met1-Thr476) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Kinase activity untested

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 3 mM DTT, 10 % glycerol

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Andere Bezeichnung: CHK1/CHEK1

Hintergrund: C85740,Chk1,rad27,CHK1 / CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtypes of chks that have so far been identified, CHK1 / CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1 / CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1 / CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1 / CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.

Molekulargewicht:

Calculated MW: 82.2 kDa

Observed MW: 78 kDa

UniProt: O35280

Pathways: p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur