EGF Protein (AA 971-1023)

Produktnummer ABIN7122799

Recombinant EGF-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für EGF Protein (AA 971-1023) (ABIN7122799)

Target: EGF (Epidermal Growth Factor (EGF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB), ELISA

Reinheit: Greater than 90 % as determined by SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 971-1023

Verwendungszweck: Recombinant Human EGF

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 0.01 EU/μg as determined by LAL test.

Biological Activity Comment: Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1-0.5 ng/mL.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 150 mM NaCl with 5 % trehalose, pH 7.4.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Haltbarkeit: 12 months

Antigendetails

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF

Hintergrund: Beta urogastrone, beta-urogastrone, EGF, EGF_HUMAN, Epidermal growth factor, HOMG4, Pro epidermal growth factor, URG, Urogastrone

Molekulargewicht: 5.7 kDa

UniProt: P01133

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation