LIF Protein

Produktnummer ABIN7199480

Dieses Recombinant LIF-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für LIF Protein (ABIN7199480)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: >90 % as determined by SDS-PAGE.

Güteklasse: MALS verified

Produktdetails

Verwendungszweck: ActiveMax® Mouse LIF Protein, Tag Free (MALS verified)

Sequenz: Ser 24 - Phe 203

Produktmerkmale: ActiveMax® Mouse LIF, Tag Free (LIF-M5219) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Phe 203 (Accession # P09056-1).

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: This protein carries no "tag". The protein has a calculated MW of 20.0 kDa. The protein migrates as 35-45 kDa under reducing (R) condition due to glycosylation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Antigendetails

Target: LIF (Leukemia Inhibitory Factor (LIF))

Andere Bezeichnung: LIF

Hintergrund: Synonyms: LIF,CDF,DIA,HILDA,MLPLI,
Description: Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

Molekulargewicht: 20.0 kDa

NCBI Accession: NP_032527

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding