LIF Protein

Produktnummer ABIN6700220

Hersteller Produkt- Nr.: 009-f01-v72-0005

Dieses Recombinant LIF-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für LIF Protein (ABIN6700220)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 98,00%

Güteklasse: Animal-Free

Produktdetails

Hersteller Produkt- Nr.: 009-f01-v72-0005

Hersteller: Rockland

Verwendungszweck: Human Leukemia Inhibitory Factor Recombinant Protein (Animal Free)

Aufreinigung: Human Leukemia Inhibitory Factor is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 98% as determined by reducing and non-reducing SDS-PAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by its ability to induce proliferation of TF-1 cells and is typically less than 0.07 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Leukemia Inhibitory Factor Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Leukemia Inhibitory Factor in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Konzentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: LIF (Leukemia Inhibitory Factor (LIF))

Andere Bezeichnung: LIF

Hintergrund:

Synonyms: Differentiation-stimulating factor (D factor), Leukocyte Inhibitory Factor, Melanoma-derived LPL inhibitor (MLPLI)

Background: Human Leukemia Inhibitory Factor (LIF) is member of the IL-6 family and is made by a variety of tissues. Human LIF signals through gp130 to activate STAT3 in human ESCs . In mouse ESCs, LIF promotes self-renewal and pluripotency, but in human cells there is no indication that LIF has the same function. Instead, FGF-basic (FGF-2) and Activin A are used to promote self-renewal and pluripotent potential. Recombinant human LIF is a non-glycosylated protein, containing 181 amino acids, with a molecular weight of 19.9 kDa.

UniProt: P15018

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding