EGF Protein

Produktnummer ABIN6699795

Hersteller Produkt- Nr.: 009-f01-c26-0100

Recombinant EGF-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für EGF Protein (ABIN6699795)

Target: EGF (Epidermal Growth Factor (EGF))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 95,00%

Güteklasse: Animal-Free

Produktdetails

Hersteller Produkt- Nr.: 009-f01-c26-0100

Hersteller: Rockland

Verwendungszweck: Human Epidermal Growth Factor Recombinant Protein (Animal Free)

Aufreinigung: Epidermal Growth Factor (EDF) is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 95% as determined by analysis by HPLC, UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-PAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of murine BALB/c 3T3 cells and is typically between 20-100 pg/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: Epidermal Growth Factor Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Epidermal Growth Factor in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 1.0 mL

Konzentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF

Hintergrund:

Synonyms: Urogastrone, URG

Background: Epidermal Growth Factor (EGF) is a growth factor that stimulates the proliferation of epithelial and epidermal cells. EGF family members are characterized by three intramolecular disulfide bonds and can bind to four different receptor tyrosine kinases known as EGFR/ErbB1, ErbB2, ErbB3, and ErbB4. Recombinant human EGF is a single, non-glycosylated protein containing 53 amino acids, with a molecular weight of 6.2 kDa.

UniProt: P01133

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation