Cyclin A Protein (GST tag)

Produktnummer ABIN6699735

Hersteller Produkt- Nr.: 009-001-r76s

Recombinant Cyclin A-Protein exprimiert in Insect cells (Sf9).

Kurzübersicht für Cyclin A Protein (GST tag) (ABIN6699735)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Insect cells (Sf9)

Applikation: Western Blotting (WB)

Reinheit: >75%

Produktdetails

Aufreinigungstag / Konjugat: Dieses Cyclin A Protein ist gelabelt mit GST tag.

Hersteller Produkt- Nr.: 009-001-r76s

Hersteller: Rockland

Verwendungszweck: CyclinA2 recombinant protein-GST fusion protein

Aufreinigung: Recombinant full-length human CyclinA2 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.

Anwendungsinformationen

Applikationshinweise:

Application Note: CyclinA2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. CyclinA2 Protein is suitable for use in Western Blot. Expect a band approximately ~ 78 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 μg/μL

Buffer: CyclinA2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Andere Bezeichnung: CCNA2

Hintergrund:

Synonyms: CCNA2, CCN1, CCNA, Cyclin-A2, Cyclin-A

Background: CyclinA2 belongs to the highly conserved cyclin family whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle and function as regulators of CDKs. CyclinA2 is an essential component of all embryonic and somatic cell cycles in mammals (1). CyclinA2 is highly expressed in the testis and certain myeloid leukemia cells (2). Cyclin A also plays a major role in the control of DNA replication (3). CyclinA2 binds and activates CDC2 or CDK2 and thus promotes both cell cycle G1/S and G2/M transitions. CyclinA2 Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.

NCBI Accession: NM_001237

Pathways: PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA