IL-15 Protein (AA 50-162) (His tag)
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- Target Alle IL-15 (IL15) Proteine anzeigen
- IL-15 (IL15) (Interleukin 15 (IL15))
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 50-162
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses IL-15 Protein ist gelabelt mit His tag.
- Applikation
- Activity Assay (AcA), Cell Culture (CC)
- Produktmerkmale
- Tag location: N-terminal His Tag
- Reinheit
- > 97 %
- Biological Activity Comment
- Interleukin 15 (IL15) is a widely expressed cytokine that is structurally and functionally related to IL2, which plays an important role in many immunological diseases. IL15 also regulates T and natural killer (NK) cell activation and proliferation. To test the effect of IL15 on cells proliferation of human T lymphocyte cells, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL15. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of Jurkat cells after incubation with IL15 for 72h observed by inverted microscope was shown in Figure 1. The dose-effect curve of IL15 was shown in Figure 2. It was obvious that IL15 significantly promoted cell proliferation of Jurkat cells. The ED50 for this effect is typically 0.7240 to 5.206ng/mL.
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Interleukin 15 (IL15) protein
IL15 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
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Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag)IL15 Spezies: Maus Wirt: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (His tag)IL15 Spezies: Human Wirt: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) proteinIL15 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag,AVI tag,Biotin)IL15 Spezies: Maus Wirt: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 15 (IL15) (AA 49-162) (Active) protein (Fc Tag,AVI tag,Biotin)IL15 Spezies: Human Wirt: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- Applikationshinweise
- Isoelectric Point: 5.1
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Konservierungsmittel
- Dithiothreitol (DTT), Other preservative, ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- IL-15 (IL15) (Interleukin 15 (IL15))
- Abstract
- IL15 Produkte
- Molekulargewicht
- 14/16/18kDa
- UniProt
- P40933
- Pathways
- JAK-STAT Signalweg, Glycosaminoglycan Metabolic Process
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