IFNA Protein (AA 24-189) (His tag)

Produktnummer ABIN6239752

not_set IFNA-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für IFNA Protein (AA 24-189) (His tag) (ABIN6239752)

Target: IFNA (Interferon alpha (IFNA))

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Applikation: Activity Assay (AcA), Cell Culture (CC)

Reinheit: > 97 %

Produktdetails

Proteineigenschaft: AA 24-189

Aufreinigungstag / Konjugat: Dieses IFNA Protein ist gelabelt mit His tag.

Produktmerkmale: Tag location: N-terminal His Tag

Biological Activity Comment: The Interferon-alpha (IFNa) proteins belong to type I interferons (IFNs) which a large subgroup of interferon proteins that help regulate the activity of the immune system. The IFNa proteins produced by leukocytes are also known as leukocyte interferon. They are mainly involved in innate immune response against viral infection. Besides, Interferon-alpha 13 (IFNA13) has been identified as an interactor of IFNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IFNa and recombinant mouse IFNA13. Briefly, IFNA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IFNA13-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IFNapAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IFNa and IFNA13 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of IFNa with IFNA13.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 9.1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: IFNA (Interferon alpha (IFNA))

Hintergrund: Alternative Names: IFNA1, IFL, LeIF D, IFN, IFN-Alpha, IFNA13, IFN-A, IFNAP22, IFN-Alpha 1b, Interferon Alpha 1b, Interferon, Leukocytic, IFN, Leukocyte, Interferon alpha-D

Molekulargewicht: 20/25kDa

UniProt: P01572

Pathways: JAK-STAT Signalweg, TLR Signalweg, Hepatitis C, Inflammasome