IL-3 Protein

Produktnummer ABIN5505701

Recombinant IL-3-Protein exprimiert in Insektenzellen.

Kurzübersicht für IL-3 Protein (ABIN5505701)

Target: IL-3 (Interleukin 3 (IL-3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Insektenzellen

Applikation: Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Reinheit: > 98 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human Interleukin-3 / IL3 protein expressed in Insect cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin-Niveau: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 0.10 - 0.30 ng/ml, corresponding to a specific activity of 0.33 - 1.0 x 10^7 units/mg, determined by the dose dependent stimulation of TF-1 cell proliferation assay. Under this assay the bioactivity is equivalent to competit

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: > 50 μg/mL

Buffer: 20 mM NaH2PO4, 0.30 M NaCl, pH 6.0

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: IL-3 (Interleukin 3 (IL-3))

Andere Bezeichnung: Interleukin-3 / IL3

Hintergrund: The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

Molekulargewicht: 15 kDa

NCBI Accession: NP_000579

Pathways: JAK-STAT Signalweg, Regulation of Carbohydrate Metabolic Process, Autophagie