NGFB Protein (AA 122-239)

Produktnummer ABIN2667381

Recombinant NGFB-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für NGFB Protein (AA 122-239) (ABIN2667381)

Target: NGFB (Nerve Growth Factor beta (NGFB))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Flow Cytometry (FACS)

Reinheit: >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Produktdetails

Proteineigenschaft: AA 122-239

Endotoxin-Niveau:

Less than 0.1 ng per μg of protein.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Assay 1: Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1.0 x 106 units/mg. Assay 2: Determined by its ability to stimulate chick E9 DRG neurite outgrowth. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1.0 x 106 units/mg.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored at -20°C or -70°C.

Antigendetails

Target: NGFB (Nerve Growth Factor beta (NGFB))

Andere Bezeichnung: beta-NGF

Hintergrund: β-NGF is a pluripotent mediator and it exists in many tissues. Secreted β-NGF can bind to tryosine kinase A receptor with higher affinity and to p75 (NTR) with low affinity. β-NGF is well known as an endogenous neurotrophic factor involved in the embryonal development, survival, and regeneration of mammalian sympathetic and sensory neurons. In adults, β-NGF is also involved in the survival and regeneration of neurons in the central nervous system. Involvement of β-NGF in the immune system has also been reported. In vitro cultured B lymphocyte can constitutively secret β-NGF, which can act as an autocrine growth and differentiation factor for B cells. β-NGF can also downregulate the production of IFN-γ by T cells. Recombinant β-NGF has been used in many clinical trials to treat different disease including Alzheimer's disease, diabetic polyneuropathy, and HIV-associated peripheral neuropathy.

Molekulargewicht: The 118 amino acid recombinant protein has a predicted molecular mass of approximately 13.5 kDa. This protein is a disulfide-linked homodimer and the predicted molecular mass is 27 kDa. The predicted N-terminal amino acid is Ser.

Pathways: NF-kappaB Signalweg, RTK Signalweg, Regulation of Cell Size