EPO Protein (AA 27-192)

Produktnummer ABIN2666935

Dieses Recombinant EPO-Protein wird in CHO Cells produziert.

Kurzübersicht für EPO Protein (AA 27-192) (ABIN2666935)

Target: EPO (Erythropoietin (EPO))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: CHO Cells

Applikation: Flow Cytometry (FACS)

Reinheit: > 98 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 27-192

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: ED50 = 0.5 - 2.5 ng/ml, corresponding to a specific activity of 0.4 - 2 x 106 units/mg, as determined by the dose dependent stimulation of TF-1 cells proliferation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: EPO (Erythropoietin (EPO))

Andere Bezeichnung: Erythropoietin

Substanzklasse: Hormone

Hintergrund: Mouse Erythropoietin (EPO) was initially cloned from mouse genomic libraries using human and monkey cDNA probes. Mouse and human EPO are 80 % identical at amino acid and nucleotide levels. EPO is a glycoprotein composed of 40-60 % carbohydrates, and its molecular weight varies from 30-34 kD depending on the carbohydrate percentage. Hypoxia induces erythropoiesis, and hypoxia inducible factor (HIF) is directly involved in EPO expression. HIF is a heterodimer (HIF-1α, HIF-2α, and β subunits) transcription factor, and it is regulated by HIF-prolyl hydroxylase (HIF-PH) that hydroxylates the α subunits, targeting them for ubiquitination and subsequent degradation. HIF-PH is a sensor of levels of iron, oxygen, and metabolic activity. High levels of HIF protein induce EPO production in the kidney and liver, and mobilization of iron to support erythropoiesis. EPO binds to the EPOR, and transcripts of this receptor have been detected in non-erythroid precursors such as endothelial cells and renal proximal epithelial cells, nevertheless, it has been published that functional erythropoietin receptor is not detected in endothelial, cardiac, neuronal, and renal cells. Several transcriptions factors play a role in the expression of EPOR such as GATA 1, Friend of GATA (Fog1), and the erythroid specific factor SCL/Tal 1. EPO has been used in the treatment of anemia associated with chronic kidney disease, cancer patients on chemotherapy, and antiviral HIV therapy.

Molekulargewicht: The 166 amino acid recombinant protein has a predicted molecular mass of approximately 18.6 kDa. The DTT-reduced and non-reduced protein migrate at approximately 35-50 kDa and 33-48 kDa by SDS-PAGE respectively. The N-terminal amino acid is Ala.

Pathways: JAK-STAT Signalweg, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity