EPO Protein (AA 27-192)

Produktnummer ABIN2666807

Dieses Recombinant EPO-Protein wird in CHO Cells exprimiert.

Kurzübersicht für EPO Protein (AA 27-192) (ABIN2666807)

Target: EPO (Erythropoietin (EPO))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Ratte

Quelle: CHO Cells

Applikation: Flow Cytometry (FACS)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 27-192

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: ED50 = 0.5 - 2.5 ng/ml, corresponding to a specific activity of 0.4 - 2.0 x 106 units/mg, as determined by induction of TF-1 cell proliferation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in 1X PBS, pH 7.2.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: EPO (Erythropoietin (EPO))

Andere Bezeichnung: Erythropoietin

Substanzklasse: Hormone

Hintergrund: Rat Erythropoietin was cloned in 1992, and it has a 79 % and 95 % homology with human and mouse EPOs, respectively. EPO is a glycoprotein composed of 40-60 % carbohydrates, and its molecular weight varies from 30-34 kD depending on the carbohydrate percentage. Hypoxia induces erythropoiesis, and hypoxia inducible factor (HIF) is directly involved in EPO expression. HIF is a heterodimeric transcription factor (HIF-1α, HIF-2α, and beta subunits), and it is regulated by HIF-prolyl hydroxylase (HIF-PH) that hydroxylates the alpha subunits targeting them for ubiquitination and subsequent degradation. HIF-PH is a sensor of levels of iron, oxygen, and metabolic activity. High levels of HIF protein induce EPO production in the kidney and liver, and mobilization of iron to support erythropoiesis. EPO binds to EPOR, and transcripts of this receptor have been detected in non-erythroid precursors such as endothelial cells and renal proximal epithelial cells, nevertheless, it has been published that functional erythropoietin receptor is not detected in endothelial, cardiac, neuronal, and renal cells. Several transcriptions factors play a role in the expression of EPOR such as GATA1, Friend of GATA (Fog1), and the erythroid specific factor SCL/Tal1. EPO has been used to treat anemia associated with chronic kidney disease, cancer patients on chemotherapy, and as an antiviral HIV therapy.

Molekulargewicht: The 166 amino acid recombinant protein has a predicted molecular mass of approximately 18.5 kDa. The DTT-reduced and non-reduced glycosylated protein migrate at approximately 35-50 kDa by SDS-PAGE. The N-terminal amino acid is Ala.

Pathways: JAK-STAT Signalweg, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity