IL-5 Protein (AA 21-133)

Produktnummer ABIN2666666

Dieses Recombinant IL-5-Protein wird in Insektenzellen exprimiert.

Kurzübersicht für IL-5 Protein (AA 21-133) (ABIN2666666)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Insektenzellen

Applikation: Multiplex Assay (MA)

Reinheit: > 98 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 21-133

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Bioactivity was measured by its property to stimulate the proliferation of BCL1 cells in a dose dependent manner. ED50 = 0.03 - 0.15 ng/ml, corresponding to a specific activity of 0.66 - 3.3 x 107 units/mg.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Andere Bezeichnung: IL-5

Hintergrund: IL-5 is a homodimeric glycoprotein that was initially identified by its ability to support the in vitro growth and differentiation of mouse B cells and eosinophils. IL-5 induces eosinophil progenitor cell proliferation, terminal differentiation, and activation. In animal models of allergic diseases or helminth infection, IL-5 induces a massive proliferation of eosinophil progenitors in the bone marrow, promotes eosinophil recruitment with eotaxins, and prolongs eosinophil survival in local tissues. IL-5 regulates genes involved in the B cell terminal differentiation. IL-5 induces CD38-activated splenic B cells to differentiate into immunoglobulin M-secreting cells and go through m to g1 class switch recombination at the DNA level, resulting in immunoglobulin G1 (IgG1) production. IL-5 binds the IL-5R complex, which consists of an IL-5Rα chain specific for IL-5 and a common β-chain that is shared by the receptors for IL-3 and GM-CSF. The alpha subunit is required for ligand-specific binding whereas association with the beta subunit results in increased binding affinity. IL-5 plays important roles in the pathogenesis of asthma, hypereosinophilic syndromes, and eosinophil-dependent inflammatory disease.

Molekulargewicht: The 113 amino acid recombinant protein has a predicted molecular mass of approximately 13.1 kDa. The DTT-reduced protein migrates between 13 to 20 kDa and the non-reduced protein at approximately 40 kDa by SDS-PAGE. The N-terminal amino acid is Met.

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour