ErbB2/Her2 Protein (AA 23-652) (His tag,Biotin)

Produktnummer ABIN2444140

Produktdetails

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 23-652

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses ErbB2/Her2 Protein ist gelabelt mit His tag,Biotin.

Marke: MABSol®,UltraLys

Sequenz: AA 23-652

Spezifität: The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Produktmerkmale: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.2 kDa. The protein migrates as 110-115 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Reinheit: >95 % as determined by reduced SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Kommentare:

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.

Referenzen

Mortensen, Skovsgaard, Okholm, Scavenius, Dupont, Rosen, Enghild, Kjems, Gothelf: "Small-Molecule Probes for Affinity-Guided Introduction of Biocompatible Handles on Metal-Binding Proteins." in: Bioconjugate chemistry, Vol. 29, Issue 9, pp. 3016-3025, (2019) (PubMed).

Vivier, Sharma, Adumeau, Rodriguez, Fung, Zeglis: "The Impact of FcγRI Binding on ImmunoPET." in: Journal of nuclear medicine : official publication, Society of Nuclear Medicine, (2019) (PubMed).

Antigendetails

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Andere Bezeichnung: Her2 (ErbB2/Her2 Produkte)

Synonyme: CD340 Protein, HER-2 Protein, HER-2/neu Protein, HER2 Protein, MLN 19 Protein, NEU Protein, NGL Protein, TKR1 Protein, Erbb-2 Protein, Neu Protein, c-erbB2 Protein, c-neu Protein, mKIAA3023 Protein, wu:fv70f10 Protein, zgc:63601 Protein, erb2 Protein, erb-b2 receptor tyrosine kinase 2 Protein, ERBB2 Protein, Erbb2 Protein, erbb2 Protein

Hintergrund: Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30 % of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .

Molekulargewicht: 70.2 kDa

NCBI Accession: NP_004439

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Skeletal Muscle Fiber Development