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Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 37-160) (Active) protein (His tag,AVI tag,Biotin) Protein

CTLA4 Spezies: Cynomolgus, Rhesusaffen Wirt: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Produktnummer ABIN2180929
$1,672.00
Zzgl. Versandkosten $45.00
Lieferung in 11 Werktagen
  • Target Alle CTLA4 Proteine anzeigen
    CTLA4
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 37-160
    Spezies
    • 30
    • 15
    • 6
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    Cynomolgus, Rhesusaffen
    Quelle
    • 24
    • 16
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    HEK-293 Cells
    Aufreinigungstag / Konjugat
    Dieses CTLA4 Protein ist gelabelt mit His tag,AVI tag,Biotin.
    Marke
    MABSol®,PrecisionAvi
    Sequenz
    AA 37-160
    Spezifität
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Produktmerkmale
    This protein carries a Avi tag (AvitagTM) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 15.9 kDa. The protein migrates as 23-26 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
    Reinheit
    >95 % as determined by SDS-PAGE.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Less than 1.0 EU per μg by the LAL method.
  • Kommentare

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handhabung
    Please avoid repeated freeze-thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
  • Target
    CTLA4
    Andere Bezeichnung
    CTLA-4 (CTLA4 Produkte)
    Synonyme
    CTLA-4, CTLA4, CD152, Cd152, Ctla-4, Ly-56, CD, CELIAC3, GRD4, GSE, IDDM12, sCTLA4, cytotoxic T-lymphocyte associated protein 4, cytotoxic T-lymphocyte-associated protein 4, CTLA4, Ctla4
    Hintergrund
    CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA-4, an inhibitory receptor for B7 Molecules. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
    Molekulargewicht
    15.9 kDa
    NCBI Accession
    XP_005574071
    UniProt
    G7PL88
    Pathways
    Cancer Immune Checkpoints
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