IL-3 Protein (AA 20-152)

Produktnummer ABIN2018100

Produktdetails

Target: IL-3 (Interleukin 3 (IL-3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 20-152

Spezies: Human

Quelle: Escherichia coli (E. coli)

Produktmerkmale: ED50 < 0.5 ng/mL, measured by a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 2 x 10^7 units/mg.
AA 20-152, expressed with an N-terminal Met.

Reinheit: > 95 % as analyzed by SDS-PAGE and HPLC.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant human Interleukin-3 (rhIL-3) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhIL-3 remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Haltbarkeit: 6 months

Antigendetails

Target: IL-3 (Interleukin 3 (IL-3))

Andere Bezeichnung: Interleukin-3 (IL-3) (IL-3 Produkte)

Hintergrund: Interleukin-3 (IL-3) is a pleiotropic cytokine belonging to the interleukin family. IL-3 shares similarities with Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) and IL-5: they all have a four-helix bundle structure, are located on the same chromosomes in both human and mouse, are produced by activated T cells, and share receptors. The IL-3/IL-5/GM-CSF receptor family members are all heterodimeric, composed of a receptor-specific alpha chain and a common beta chain. IL-3 is also called multi-colony stimulating factor since it stimulates the development and colony formation of multiple lineages of hematopoietic cells by activating intracellular pathways such as Ras-Raf-ERK and JAK/STAT. IL-3 inhibits apoptosis and promotes cell survival by targeting the anti-apoptotic bcl-2 gene family. Recombinant human Interleukin-3 (rhIL-3) produced in E. coli is a single non-glycosylated polypeptide chain containing of 134 amino acids. A fully biologically active molecule, rhIL-3 has a molecular mass of 15.2 kDa analyzed by non-reducing SDS-PAGE.
Synonyms: MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor

Molekulargewicht: 15.2 kDa, observed by non-reducing SDS-PAGE.

UniProt: P08700

Pathways: JAK-STAT Signalweg, Regulation of Carbohydrate Metabolic Process, Autophagie