Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/mL, corresponding to a specific activity of > 5.0 x 10^8 IU/mg.
Reinheit
> 97 % by SDS-PAGE and HPLC analyses.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
< 1 EU/μg of rMuIL-1μ as determined by LAL method.
IL1A
Spezies: Ratte
Wirt: Escherichia coli (E. coli)
Recombinant
>95 % as determined by reducing SDS-PAGE.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
Handhabung
Avoid repeated freeze/thaw cycles.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C.
Interleukin-1 alpha (IL-1a) is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1a and IL-1b binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1b is a secreted cytokine, IL-1a is predominantly a cell-associated cytokine. Synonyms: Interleukin-1 Alpha, Mouse,
Molekulargewicht
17.9 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids.